rebaudioside d (ChromaDex)
93
Structured Review
ChromaDex
rebaudioside d
Rebaudioside D, supplied by ChromaDex, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rebaudioside+d/Rebaudioside+D/us12404499-1348-54-56
Average 93 stars, based on 1 article reviews
Rebaudioside D, supplied by ChromaDex, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rebaudioside+d/Rebaudioside+D/us12404499-1348-54-56
Average 93 stars, based on 1 article reviews
rebaudioside d - by Bioz Stars,
2026-10
93/100 stars
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Activity Assay:Article Title: Engineered glycosyltransferases and steviol glycoside glucosylation methods Article Snippet: The reaction was performed at 30° C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 1-4 h. The reaction described above was quenched with 0.5 volume/volume acetonitrile with 0.2% formic acid and precipitated by centrifugation. .. TABLE 5.1 Circular Permuted Round 3.2 Variants First Amino Acid Position SEQ ID NO: (nt/aa) (Relative to SEQ ID NO: 32) 75/76 70 77/78 71 79/80 72 81/82 73 83/84 74 85/86 75 87/88 169 89/90 170 91/92 171 93/94 174 95/96 194 97/98 198 99/100 259 101/102 260 103/104 370 105/106 401 107/108 403 Determination of Specific Activity of Purified Circular Permuted GTs on Article Title: Engineered glycosyltransferases and steviol glycoside glucosylation methods Article Snippet: The reaction was performed at 30° C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 0.5-2 h. The reaction was quenched by adding 50 μL acetonitrile with 0.2% formic acid and precipitated by centrifugation. .. Shake Flask Powder Characterization Assay and Analysis for Glucosyl Transfer from ADP-Glucose to Stevioside or Article Title: Engineered glycosyltransferases and steviol glycoside glucosylation methods Article Snippet: .. Agilent RapidFire Conditions Buffer A 0.1% formic acid in LC/MS grade water; 1.5 mL/min flow rate Buffer B 0.1% formic acid in LC/MS grade methanol; 0.5 mL/min flow rate Aqueous wash Water Organic wash Acetonitrile SPE cartridge Agilent RapidFire cartridge A (C4) RF state 1 600 ms RF state 2 2500 ms RF state 3 0 RF state 4 5000 ms RF state 5 1000 ms Agilent Jet Stream source parameters Drying gas 325° C. temperature Drying gas flow 7 L/min Nebulizer pressure 50 psi Sheath gas 300° C. temperature Sheath gas flow 12 L/min Capillary voltage +4000 V Nozzle voltage +500 V Agilent 6470 Triple Quadrupole MRM parameters Frag- Compound Q1 Q3 Dwell mentor CE CAV Stevioside 827.4 665.3 50 150 50 5 RebA 989.5 827.5 50 300 60 5 RebD or RebI 1151.7 827.5 50 285 55 5 RebM 1313.7 827.5 50 350 70 5 Shake Flask Powder Characterization Assay and Analysis for Glucosyl Transfer from ADP-Glucose to Stevioside or Purification:Article Title: Engineered glycosyltransferases and steviol glycoside glucosylation methods Article Snippet: The reaction was performed at 30° C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 1-4 h. The reaction described above was quenched with 0.5 volume/volume acetonitrile with 0.2% formic acid and precipitated by centrifugation. .. TABLE 5.1 Circular Permuted Round 3.2 Variants First Amino Acid Position SEQ ID NO: (nt/aa) (Relative to SEQ ID NO: 32) 75/76 70 77/78 71 79/80 72 81/82 73 83/84 74 85/86 75 87/88 169 89/90 170 91/92 171 93/94 174 95/96 194 97/98 198 99/100 259 101/102 260 103/104 370 105/106 401 107/108 403 Determination of Specific Activity of Purified Circular Permuted GTs on Article Title: Engineered glycosyltransferases and steviol glycoside glucosylation methods Article Snippet: The reaction was performed at 30° C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 1-18 h. The reaction described above was quenched with 0.5 volume/volume acetonitrile with 0.2% formic acid and precipitated by centrifugation. .. First, 5 μL purified protein was diluted in 100 μL total reaction volume of 50 mM Tris-HCl buffer, pH 7.5, 3 mM MgCl2, with 1 mM Variant Assay:Article Title: Engineered glycosyltransferases and steviol glycoside glucosylation methods Article Snippet: The reaction was performed at 50° C. in a Thermotron® shaking incubator at 300 RPM for 4 h, and the reaction was quenched by adding 10 μL assay to 90 μL acetonitrile with 0.2% formic acid and precipitated by centrifugation. .. The resulting powder, containing the variant SEQ ID NO: 548, was assayed relative to the SEQ ID NO: 500 variant, with 2.5 μL lysate added to a 100 μL total reaction volume containing 50 mM potassium phosphate buffer, pH 7, 3 mM MgCl2, 2.5% v/v ethanol with 1 mM stevioside (Chromadex, >94% purity) or Article Title: Engineered glycosyltransferases and steviol glycoside glucosylation methods Article Snippet: .. Agilent RapidFire Conditions Buffer A 0.1% formic acid in LC/MS grade water; 1.5 mL/min flow rate Buffer B 0.1% formic acid in LC/MS grade methanol; 0.5 mL/min flow rate Aqueous wash Water Organic wash Acetonitrile SPE cartridge Agilent RapidFire cartridge A (C4) RF state 1 600 ms RF state 2 2500 ms RF state 3 0 RF state 4 5000 ms RF state 5 1000 ms Agilent Jet Stream source parameters Drying gas 325° C. temperature Drying gas flow 7 L/min Nebulizer pressure 50 psi Sheath gas 300° C. temperature Sheath gas flow 12 L/min Capillary voltage +4000 V Nozzle voltage +500 V Agilent 6470 Triple Quadrupole MRM parameters Frag- Compound Q1 Q3 Dwell mentor CE CAV Stevioside 827.4 665.3 50 150 50 5 RebA 989.5 827.5 50 300 60 5 RebD or RebI 1151.7 827.5 50 285 55 5 RebM 1313.7 827.5 50 350 70 5 Shake Flask Powder Characterization Assay and Analysis for Glucosyl Transfer from ADP-Glucose to Stevioside or Expressing:Article Title: Engineered glycosyltransferases and steviol glycoside glucosylation methods Article Snippet: The following reaction conditions were used: 50 mM Tris-HCl buffer, pH 7.5, 3 mM MgCl2, 30° C. in a Thermotron® titre-plate shaker with 300 RPM shaking for 1 h. The reactions were quenched by diluting the assay 1:10 and then quenching with 50 μL/well acetonitrile with 0.2% formic acid, centrifuged 10 m at 4° C., and the supernatants were diluted 1:10 in water and analyzed by HPLC-MS/MS as described in Example 1, Table 1.1. .. HTP Assay for Glucose Transfer from ADP-Glucose to Targeted Proteomics:Article Title: Engineered glycosyltransferases and steviol glycoside glucosylation methods Article Snippet: .. Agilent RapidFire Conditions Buffer A 0.1% formic acid in LC/MS grade water; 1.5 mL/min flow rate Buffer B 0.1% formic acid in LC/MS grade methanol; 0.5 mL/min flow rate Aqueous wash Water Organic wash Acetonitrile SPE cartridge Agilent RapidFire cartridge A (C4) RF state 1 600 ms RF state 2 2500 ms RF state 3 0 RF state 4 5000 ms RF state 5 1000 ms Agilent Jet Stream source parameters Drying gas 325° C. temperature Drying gas flow 7 L/min Nebulizer pressure 50 psi Sheath gas 300° C. temperature Sheath gas flow 12 L/min Capillary voltage +4000 V Nozzle voltage +500 V Agilent 6470 Triple Quadrupole MRM parameters Frag- Compound Q1 Q3 Dwell mentor CE CAV Stevioside 827.4 665.3 50 150 50 5 RebA 989.5 827.5 50 300 60 5 RebD or RebI 1151.7 827.5 50 285 55 5 RebM 1313.7 827.5 50 350 70 5 Shake Flask Powder Characterization Assay and Analysis for Glucosyl Transfer from ADP-Glucose to Stevioside or |